<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Zinselmeyer, B. H.</style></author><author><style face="normal" font="default" size="100%">Mackay, S. P.</style></author><author><style face="normal" font="default" size="100%">Schatzlein, A.G.</style></author><author><style face="normal" font="default" size="100%">Uchegbu, I. F.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The lower-generation polypropylenimine dendrimers are effective gene-transfer agents</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmaceutical Research</style></secondary-title><short-title><style face="normal" font="default" size="100%">Pharm. Res.</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Gene Transfer Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Comparative Study</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA/administration &amp; dosage/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Drug Delivery Systems/methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Escherichia coli</style></keyword><keyword><style  face="normal" font="default" size="100%">Human</style></keyword><keyword><style  face="normal" font="default" size="100%">Imines/*administration &amp; dosage/chemistry/toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Models, Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">Polypropylenes/*administration &amp; dosage/chemistry/toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Support, Non-U.S. Gov't</style></keyword><keyword><style  face="normal" font="default" size="100%">Tumor Cells, Cultured</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jul</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">7</style></number><volume><style face="normal" font="default" size="100%">19</style></volume><pages><style face="normal" font="default" size="100%">960-7</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">OBJECTIVE: To evaluate polypropylenimine dendrimers (generations 1-5: DAB 4, DAB 8, DAB 16, DAB 32, and DAB 64) as gene delivery systems. METHODS: DNA binding was evaluated by measuring the reduced fluorescence of ethidium bromide, and molecular modelling of dendrimer-DNA complexes also was performed. Cell cytotoxicity was evaluated against the A431 cell line using the MTT assay. In vitro transfection was evaluated against the A431 cell line using the beta-galactosidase reporter gene and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulphate (DOTAP) served as a positive control. RESULTS: Molecular modeling and experimental data revealed that DNA binding increased with dendrimer generation. Cell cytotoxicity was largely generation dependent, and cytotoxicity followed the trend DAB 64 &gt; DAB 32 &gt; DAB 16 &gt; DOTAP &gt; DAB 4 &gt; DAB 8, whereas transfection efficacy followed the trend DAB 8 = DOTAP = DAB 16 &gt; DAB 4 &gt; DAB 32 = DAB 64. CONCLUSION: The generation 2 polypropylenimine dendrimer combines a sufficient level of DNA binding with a low level of cell cytoxicity to give it optimum in vitro gene transfer activity.</style></abstract><accession-num><style face="normal" font="default" size="100%">12180548</style></accession-num><notes><style face="normal" font="default" size="100%">2.609</style></notes><auth-address><style face="normal" font="default" size="100%">Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland.</style></auth-address><label><style face="normal" font="default" size="100%">ASPAPER</style></label></record></records></xml>